Fig. 5: PKARIIβ knockdown modifies p-PKA signaling in the striatum of WT and RC mice. | Communications Biology

Fig. 5: PKARIIβ knockdown modifies p-PKA signaling in the striatum of WT and RC mice.

From: R1441C and G2019S LRRK2 knockin mice have distinct striatal molecular, physiological, and behavioral alterations

Fig. 5

a Striatal sections of AAV-eGFP-shcontrol and AAV-eGFP-shPKARIIβ injected WT and RC mice. Scale bar, 50 μm. b Western blot analysis of striatum of WT mice injected with AAV-sh-control and AAV-sh-PKARIIβ viral constructs probed for PKARIIβ. c Quantification of PKARIIβ from b normalized to β-actin. Summary graphs represent the mean, while error bars SEM, **p < 0.01, unpaired t-test (n = 5). d Western Blot analysis of striatum of WT mice injected with, AAV-sh-control and AAV-sh-PKARIIβ probed for PKARIIβ, pPKARIIβ, p-S845GluA1, GluA1 and β-actin. e Western blot analysis of striatal extracts from WT and RC mice injected with AAV-sh-control and AAV-sh-PKARIIβ viral constructs probed for PKARIIβ, and gapdh. f Quantification of PKA bands normalized to gapdh. Summary graphs represent the mean, while error bars SEM, *p < 0.05, **p < 0.01, Tukey post hoc following one-way ANOVA (n = 3).

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