Fig. 2: Efficient A-to-G conversion by Cas12a variant-mediated ABEs for non-canonical PAMs.
From: Multiplexed base editing through Cas12a variant-mediated cytosine and adenine base editors
a Schematic of A-to-G editing on targets with non-canonical PAMs by Cas12a variant-mediated ABEs. Efficiencies of Cas12a variant-mediated ABEs, including dCas12a-, enCas12a-, RR-, and RVR-ABE8e-V106W, for canonical TTTV PAM (b) and non-canonical PAMs (c) in HKE293T cells (n = 3). d Average efficiencies of Cas12a variant-mediated ABEs at 14 sites in HEK293T cells with individual PAMs. Related to b, c, and Supplementary Fig. 10. e Efficiencies of Cas12a variant-mediated ABEs for canonical TTTV PAM and non-canonical PAMs, TCCA and CTTC, in porcine embryos (n ≥ 3). Values and error bars for b, c, and e represent the mean and SEM, respectively. For d, the boxplots show the median, first quartile, and third quartile, and whiskers represent the maximum and minimum values. Statistical significance was calculated by unpaired two-tailed Mann–Whitney test. ns (not significant), p ≥ 0.05; *p < 0.05.
