Fig. 5: Specificity of Cas12a variant-mediated base editors in HEK293T cells.
From: Multiplexed base editing through Cas12a variant-mediated cytosine and adenine base editors
Heat maps showing the frequencies of C-to-non-T (C-to A or C-to-G) conversion for CBEs (a) and A-to-non-G (A-to-C or A-to-T) for ABEs (b) at five selected sites with TTTV PAM (n = 3). c Indels frequencies for indicated CBEs and ABEs at the tested sites (n = 3). Heat maps showing the Cas12a-dependent off-target activity of indicated CBEs (d) and ABEs (e) at the predicted off-target sites (n = 3). The spacer sequences of off-target sites are shown with mismatches marked in lowercase and PAM marked in red and in italics. Values and error bars represent the mean and SEM, respectively. All heatmaps show the mean of three independent biological replicates.
