Fig. 3: Screening of SREBP target genes for terpenoid and lipid metabolism. | Communications Biology

Fig. 3: Screening of SREBP target genes for terpenoid and lipid metabolism.

From: The bHLH-zip transcription factor SREBP regulates triterpenoid and lipid metabolisms in the medicinal fungus Ganoderma lingzhi

Fig. 3

a KEGG analysis of SREBP target genes. Clusters for terpenoid and lipid metabolism were highlighted by the red line. b Schematic diagram showing the genomic locations of SREBP target genes that involved in terpenoid and lipid metabolism, and corresponding matched DNA sequence, promoter regions, and fitted motif. The conserved SREBP binding sequence was highlighted in blue shadow. c SREBP can bind to the DNA sequences in the targets promoter by EMSA. Line 1: the labelled DNA probe control. The labelled DNA probe was preincubated with 0.6 μg SREBP-bHLH protein (Line 2), and an unlabelled competitor DNA probe (200× labelled DNA probe) was added (Line 3). Arrows indicate positions of protein-bound (red) and free (black) DNA bands.

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