Fig. 6: 3D analysis of NMJs during postnatal development. | Communications Biology

Fig. 6: 3D analysis of NMJs during postnatal development.

From: Three-dimensional mapping reveals heterochronic development of the neuromuscular system in postnatal mouse skeletal muscles

Fig. 6

a 3D distributions of NMJs in the gastrocnemius at different time points (P0, P3, P6, P9, and adulthood); scale bar: 200 μm. The images in the white frames are enlarged and shown in the second row; scale bar: 100 μm. The images in the third row show the segmentation of NMJs. b–d The quantification of NMJs in the gastrocnemius (b), biceps brachii (c), and cleidomastoid (d) at different time points (n = 5 independent animals for each muscle at each time point). All values are presented as the means ± SEM; statistical significance in (b-d) (n.s. represents not significant and ***P < 0.001) was assessed using one-way ANOVA followed by Tukey post hoc test.

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