Fig. 6: DivIC interacts with the cell wall in a teichoic acid-dependent manner.
a Fluorescence of Cy2-labelled DivICex measured after 5 min incubation with purified cell walls or PG from SH1000. Cy2-labelled DivICex fluorescence was determined in the supernatants after centrifugation to determine the levels of cell wall- or PG-unbound protein. Mean and standard deviations of three independent experiments are shown. P-values were determined by two-tailed t-test corrected for multiple comparison using the Holm-Å Ãdák method. b Cell walls (0.25 mg/ml) from SH1000, ΔtarO (SJF5289) mutant or complemented ΔtarO (SJF5396) were incubated with Cyt2-labelled DivIC (500 nM) for 5 min and fluorescence measured in the supernatant after centrifugation. Mean and standard deviations of three independent repeats are shown. P-values were calculated by two-tailed t-test. c Localisation of IPTG-induced GFP-DivIC in SH1000 (SJF5398) and ΔtarO (SJF5395) cells grown to mid exponential phase and incubated with HADA for 30 min to label PG. Scale bars, 2 μm. d FR between GFP-DivIC fluorescence signal at the septum versus the cell periphery. Each circle indicates a single cell, lines are mean of an n = 100. P-values were determined by Mann–Whitney U-tests. All images are average intensity projections of z stacks of cells with incomplete septa. Data is representative of two independent experiments. (*P < 0.05, **P < 0.01, ***P < 0.005, ****P < 0.0001).
