Fig. 5: Pharmacological modulation of image-based single cell calcium flux for 2D and 3D iDopas/iAstros cellular co-cultures. | Communications Biology

Fig. 5: Pharmacological modulation of image-based single cell calcium flux for 2D and 3D iDopas/iAstros cellular co-cultures.

From: High throughput 3D gel-based neural organotypic model for cellular assays using fluorescence biosensors

Fig. 5

a, b Example of ROIs from every pharmacological experiment with each physiological variations; Basal, Basal with drug, Evoked, Evoked with drug; for the 2D and 3D co-cultures models, respectively. Quantitative measurements of calcium peak frequency (c, d) and mean peak amplitudes from each ROIs (e, f), the spontaneous and evoked calcium flux, for 2D and 3D co-cultures for each drug tested. Minimal of three ROIs from each well and three technical replicates, each with 3 wells per group were plotted as bar graph. Error bar s.e.m., Statistics: Two-way ANOVA with post hoc turkey test between groups.

Back to article page