Fig. 7: Image-based quantitation and pharmacological modulation of dopamine release by genetically encoded dopamine sensor in iDopas/iAstros 2D and 3D co-cultural model.

The genetically encoded dopamine biosensor, hSyn-dLight1.2, was used to quantitate release of dopamine neurotransmitter at the synaptic clefts in the 2D and 3D gel neural co-culture models. a Representative images of expression of ChrimsonR via td-Tomato fluorescence, dopamine neurotransmitter release via dLight1.2 as green puncta at the synapses, for both basal and optical stimulation, and with or without Apomorphine, the D2-receptor agonist treatment. b, c Quantitative estimation of mean fluorescent intensity from multiple fields of views for 2D system (n = 9 wells) and from entire z-stack along with multiple fields of views for 3D system (n = 9 wells) were accumulated and presented as bar plots. Quantitation of dopamine release by measuring green fluorescence from dLight1.2 biosensor in 2D (b) and 3D (c) models, after 30 min min treatment with apomorphine, metoclopramide, D-serine and ketamine at 10 µM, and morphine at 15 µM. Scale bar 50 µm. Error bar s.e.m. three technical replicates, with n = 3 wells each. Statistics: Two-way ANOVA with post hoc turkey test between groups.