Fig. 8: Successful microglia targeting, but aggregate formation in SCA1-Tg mice by intravenous administration of AAV-PHP.B. a Diagram showing the AAV construct and experimental procedure. | Communications Biology

Fig. 8: Successful microglia targeting, but aggregate formation in SCA1-Tg mice by intravenous administration of AAV-PHP.B. a Diagram showing the AAV construct and experimental procedure.

From: Development of microglia-targeting adeno-associated viral vectors as tools to study microglial behavior in vivo

Fig. 8

SCA1-Tg mice at 36 weeks of age received intravenous infusion of AAV-PHP.B.Iba1.miR-9.T.miR-129-2-3p.T (dose: 1.5 Ɨ1012 vg/mouse). Three weeks after the viral injection, brain sections were produced and analyzed by double immunolabeling for GFP and Iba1. (b-d) Immunofluorescent images of a sagittal section (lobules II and III) from the SCA1-Tg mouse (b) and the magnification of square regions (c, d). Note aggregation of GFP in microglia in SCA1-Tg mouse cerebellum. Scale bars, 20 Ī¼m. GL; granule cell layer, i.v.; intravenous injection, ML; molecular layer, SCA1-Tg; spinocerebellar ataxia type 1-transgenic.

Back to article page