Fig. 2: Mobile fraction, distribution, and stability of NET subunits.

a Mobile fraction of NET determined by FRAP microscopy. Data points represent averages of ten cells (± SD), which were fitted by a one-phase association curve (Equ. 1). b NET oligomerization displays a slight transporter density-dependent behavior. Densities of 261 cells measured under control conditions were plotted against the average oligomeric state, resulting in a significant correlation (Spearman r: 0.1898, P < 0.01). c Sketch showing two potential scenarios. i) Scenario 1: dimers are stable; repeated runs result in a decreased number of active fluorophores but no altered distribution. ii) Scenario 2: dimers exchange subunits; the exchange of bleached (white) and unbleached (green) dimers results in an increasing fraction of apparent monomers. d Repeated TOCCSL runs on the same cell indicate that a small portion of NET dimers exchange subunits over time. A total of 20 cells were repeatedly measured three times over the course of 20 min. Data show means ± SD. e Average oligomeric state of NET molecules for each of the repeatedly measured cells.