Fig. 3: Effect of cholesterol and PIP₂ on NET oligomerization and function.

Oxidation (a) and depletion (b) of membrane cholesterol did not affect NET oligomerization. Each data point shows an independent experiment with TOCCSL runs on ten cells. Bars represent means ± SD. c NET oligomerization after PIP₂ depletion with the direct PLC activator m-3M3FBS and after treatment with its inactive analog o-3M3FBS. Each data point represents an independent experiment with TOCCSL runs on ten cells. Bars represent means ± SD. d PIP₂ depletion resulted in a significantly decreased average oligomeric state (**P < 0.01). e Surface density of mGFP-hNET after m-3M3FBS or o-3M3FBS pretreatment was statistically indifferent. f NET-mediated substrate efflux over time, induced with 10 µM d-methamphetamine after 6 min. The data were derived from five independent experiments (mean ± SD). Single data points are shown in Supplementary Fig. 6a. g Total d-methamphetamine-induced substrate efflux was decreased under PIP₂-depleted conditions. Bars represent mean efflux (±SD) of five experiments, normalized to basal efflux.