Fig. 7: Temporal knockdown of KRT31/KRT34 expressions in vivo suppressed hair growth.
From: Keratin-mediated hair growth and its underlying biological mechanism
a Graphical representation of KRT31 and KRT34 mRNA expressions in mice on day 7 after KRT31/KRT34 knockdown. Control, mice injected with negative control siRNA-loaded lipofectamine; siRNA, KRT31/KRT34 knockdown mice; siRNA+KRT, KRT31/KRT34 knockdown, and hair keratin-injected mice; *P,0.05, indicates a difference between control and experimental groups. (n = 4, in 4 mice; mean ± standard deviation (s.d.)). b Images of hair growth on the back skin of mice on day 3, day 7, day 10, and day 14 after KRT31/KRT34 siRNA transfection. c Histological images of the back skin of mice on day 7 after KRT31/KRT34 siRNA transfection and intradermal injection of keratin. Graphical representation and quantification of hair follicle formation in skin sections of mice (n = 36 sections, in 8 mice; mean ± standard deviation (s.d.)). *P,0.01, indicates a difference between control group and experimental groups. Scale bars, 200 μm. d Immunohistochemical images of the back skin of mice on day 7 after KRT31/KRT34 siRNA transfection and intradermal injection of keratin; β-catenin, red; P-cadherin, green; DAPI, blue. Scale bars, 20 μm. e Histological images of the back skin of mice on day 14 after KRT31/KRT34 siRNA transfection and intradermal injection of keratin. Graphical representation and quantification of hair follicle formation in skin sections of mice (n = 23 sections, in five mice; mean ± standard deviation (s.d.)). *P,0.01, indicates a difference between control group and experimental groups. Scale bars, 20 μm.
