Table 1 Enumeration of phage progeny released from strains GBJ002(GIL01), GBJ002(pDG) and GBJ002(pDG7).

From: A small bacteriophage protein determines the hierarchy over co-residential jumbo phage in Bacillus thuringiensis serovar israelensis

Time after MMC induction

Strain

0 min

30 min

1 h

4 h

GBJ002(GIL01)

8.3 ×106 ± 2.9 ×106

4.3 ×107 ± 4.7 ×106

1.7 ×1010 ± 9.0 ×109

NA

GBJ002(GIL01)*

1.1 ×107 ± 3.9 ×106

3.3 ×107 ± 9.4 ×106

1.9 ×1010 ± 1.5 ×1010

NA

GBJ002(pDG)

7.5 ×104 ± 5.0 ×103

1.5 ×105 ± 5.5 ×104

2.7 ×105 ± 1.1 ×105

1.8 ×106 ± 5.5 ×105

GBJ002(pDG7)

1.7 ×104 ± 5.0 ×102

3.5 ×104 ± 1.5 ×104

2.0 ×104 ± 5.0 ×102

1.5 ×103 ± 5.0 ×102

GBJ002(pDG)*

0

0

0

0

  1. Strain GBJ002(GIL01) contains both the GIL01 and pBtic235 prophages whereas, strains GBJ002(pDG) and GBJ002(pDG7) carry only the pBtic235 prophage. The lytic cycle of both prophages was induced by adding MMC (100 ng mL−1) to Bacillus cultures in the early exponential phase. Dilutions of the culture supernatants taken at 0 min, 30 min, 1 h and 4 h after MMC induction were spotted onto lawns of recipient strain HER1410 and the plaques formed were counted (PFU mL−1) to determine the number of phage progeny produced after the different times. An asterisk (*) indicates samples vortexed for 10 s. Not assessed (NA).
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