Fig. 1: ANGPTL2 is expressed at early stages of AoV development in WT mice.

a–d Representative views of in situ hybridizations with Angptl2 RNA probes on WT mice embryos at E9.5, E10, E11 and E11.5. e, f Representative views of in situ hybridizations with Angptl2 RNA probes on Angptl2-KD mice embryos at E9.5 and E10.5. g, h Higher magnifications of the heart region at E11 and E11.5; The outflow tract (oft) is indicated by black arrows. i, j Representative ANGPTL2 and CD31 protein expression by immunofluorescence in cardiac sections from (i) E14.5 and from (j) E18 embryo from WT mice. At a higher magnification (×63), white arrows show the expression of ANGPTL2 on the edge of the leaflet and indicate cells co-expressing ANGPTL2 and CD31 in E14.5 embryo. k Representative ANGPTL2 and CD31 protein expression by immunofluorescence in cardiac sections from 2-month old WT mice. l Higher magnification (×63) of ANGPTL2 and CD31 expression in AoV leaflets of WT mice: ANGPTL2 is not co-expressed with CD31-positive cells (indicated by white arrows). All images are representative of n = 3 independent experiments. DAPI staining was used to visualize cell nuclei.