Fig. 7: Circ-CREBBP maintains the survival of sperm via the PI3K/Akt pathway. | Communications Biology

Fig. 7: Circ-CREBBP maintains the survival of sperm via the PI3K/Akt pathway.

From: Circ-CREBBP inhibits sperm apoptosis via the PI3K-Akt signaling pathway by sponging miR-10384 and miR-143-3p

Fig. 7

a Western blot assays were used to evaluate the total protein levels of PI3K/Akt and apoptosis pathway members MCL1, CREBBP, CREB1, BCL2, BAX, and CASP3, the ubiquitination level of MCL1 and the phosphorylation levels of CREB1 in sperm incubated with EV, EV-miR-10384/EV-miR-143-3p mimic, and EV-miR-10384/EV-miR-143-3p inhibitor. b Immunofluorescence data showed that MCL1 and BAX and CREBBP and CREB1 were mainly colocalized in sperm tails. c The mRNA expression of MCL1, CREBBP and CREB1 in sperm incubated with EV (NC) or Si-circ-CREBBP was detected by qPCR. Data are presented as the mean ± SEM. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001 (Student’s t test), n = 3 for each group. d Western blot assays were used to detect the protein levels of PI3K/Akt and apoptosis pathway members MCL1, CREBBP, CREB1, BCL2, BAX, and CASP3, the phosphorylation levels of CREB1 and the ubiquitination level of MCL1 in sperm incubated with EV (NC) or EV-Si-circ-CREBBP.

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