Fig. 1: An in vitro platform that mimics HCM myocardial stiffness.
a Schematic indicating structural-functional link between the L-type calcium channel, cytoskeletal network and mitochondria in wt cardiac myocytes. Targeted linker sites (1) L-type calcium channel α-interaction domain (AID), (2) interaction site between the L-type calcium channel β2 subunit and AHNAK, (3) F-actin, (4) β-tubulin, and (5) β1 integrin. b Compressive stiffness (in kPa) of polyacrylamide (PA) hydrogels under manufactured conditions (soft and stiff), including mean ± SEM. c Compressive stiffness (in kPa) of wt cardiac myocytes plated on soft and stiff hydrogels, including mean ± SEM. Representative images and quantitated data points for wt cardiac myocytes plated on soft and stiff hydrogels stained for lamin A (d, e), vinculin (f, g), and integrin (h, i), including mean ± SEM. n = number of cells, N = number of hydrogels. Statistical significance determined by Mann Whitney tests.
