Fig. 5: Distortion of the oviduct transcriptome in Tgfbr2^cKO oviduct.

a Volcano plot illustrating the differential expression genes in the oviductal cells (at 13 h post-hCG) of Tgfbr2^fl/fl and Tgfbr2^cKO mice determined by RNA-seq analysis (n = 3 mice in each group). The pink spots denote the transcripts that were validated by qRT-PCR. b qRT-PCR validating changes in representative transcripts selected from RNA-seq data (n = 3 independent experiments). Bars indicate the mean ± SD. Each data point represents a single mouse. Statistical analysis was performed by a two-tailed unpaired Student’s t-test. **P < 0.01 and ***P < 0.001. c Bar graph showing the enriched GO/KEGG terms associated with the significantly upregulated transcripts in oviducts isolated from the Tgfbr2^cKO mice identified by RNA-seq, with the color indicating P value (n = 3 mice in each group). d, e GSEA plots illustrating enrichment of gene sets of cell adhesion molecules (d) and NF-kappa B signaling pathway (e) in oviductal cells of Tgfbr2^fl/fl and Tgfbr2^cKO mice (n = 3 mice in each group). f Heatmap showing differences between the Tgfbr2^fl/fl and Tgfbr2^cKO oviducts in the expression of a group of transcripts involved in various processes (n = 3 mice in each group).