Fig. 7: AHA2 expression in developing seeds is confined to the micropylar endosperm region, the outer seed coat, and the endothelium. | Communications Biology

Fig. 7: AHA2 expression in developing seeds is confined to the micropylar endosperm region, the outer seed coat, and the endothelium.

From: The evolution of plant proton pump regulation via the R domain may have facilitated plant terrestrialization

Fig. 7

Confocal images of AHA2 expression in developing mCitAHA2 seeds. Yellow: mCitrine-AHA2. Red: autofluorescence. Scale bar: 100 µm. a Maximum intensity projection of AHA2 in early-developing seeds. AHA2 is expressed exclusively at the micropylar endosperm region. b Top panel: confocal image of an optical section of mature green seed. Bottom panel: maximum intensity projection of AHA2 distribution in mature green seed. The images show that at the mature stage AHA2 is highly expressed in the seed coat. c Confocal images of a mature green seed cut with a vibratome. Yellow arrows point to the endothelium and outer seed coat where AHA2 is expressed. d Confocal images of an embryo at the mature green stage show that AHA2 is expressed mostly in the radicle.

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