Fig. 3: acdh-1, kat-1 and elo-6 are essential for neuroprotection provided by HA-114.
a Volcano plot of RNA-Seq data of N2 worms fed with Lacticaseibacillus rhamnosus HA-114 and compared to worms fed with OP50. The data for all genes were plotted as log2 fold change versus -log10 of the adjusted p value. Gene ontology (GO) term analysis of the differentially overexpressed genes induced by HA-114 treatment. The GO term analysis was performed with PANTHER 11 by using C. elegans genes as background. Only the biological process terms with an enrichment of p value <0.01 are shown in this figure. Data: GEO accession: GSE189988; SRA study: SRP348888. b L. rhamnosus HA-114 significantly increase GFP expression in pacdh-1::GFP worms when compared to the same worms fed with OP50. Scale bar = 100 μm. Deletion of acdh-1 (c), kat-1 (d), and elo-6 (e) altered the beneficial effect of HA-114. For fluorescence quantification (b), an unpaired t test was performed and n are indicated in the figure. For paralysis assays (c–e), curves were generated and compared using the log-rank (Mantel–Cox) test. c: FUSS57Δ;acdh-1(ok1489) on OP50 n = 250; FUSS57Δ;acdh-1(ok1489) on HA-114 n = 190. d: FUSS57Δ;kat-1(tm1037) on OP50 n = 437; FUSS57Δ;kat-1(tm1037) on HA-114 n = 463. e: FUSS57Δ;elo-6(gk233) on OP50 n = 331; FUSS57Δ;elo-6(gk233) on HA-114 n = 285. For boxplots, minimum, first quartile, median, third quartile, and maximum are shown.
