Fig. 5: Lacticaseibacillus rhamnosus HA-114 modulate lipid accumulation in worm age-dependent neurodegeneration models.
a Representative images of worms fed with OP50, L. rhamnosus HA-114, L. rhamnosus HA-111 or L. rhamnosus R0011 and stained with Oil Red O. N2 worms show a basal level of lipid accumulation. b Quantification of Oil Red O show increased fat accumulation in worms expressing FUSS57Δ, TDP-43A315T, and polyQ expansion (40Q and 67Q) when compared to N2. Worms fed with HA-114 showed a significant decrease of lipid accumulation, while both HA-111 and R0011 rhamnosus strains failed to decrease fat accumulation in all models. For the Oil Red O quantification graphs (b), Brown–Forsythe and Welch ANOVA were performed and n are indicated in the figure. Scale bar = 100 μm. For boxplots, minimum, first quartile, median, third quartile, and maximum are shown.
