Fig. 6: Impaired β-oxidation contributes to neurodegeneration and shortens lifespan.
Worms were monitored from the adult stage until death. a acs-20(tm3278) and FUSS57Δ;acs-20(tm3278) worms have shorter lifespan than N2 and FUSS57Δ; animals. b Etomoxir (40 μM), a cpt-1/CPT1 inhibitor, shorten lifespan of FUSS57Δ, acs-20(tm3278) and FUSS57Δ;acs-20(tm3278) worms when compared to N2. c acs-20(tm3278), FUSS57Δ;acs-20(tm3278), acdh-1(ok1489) and FUSS57Δ;acdh-1(ok1489) animals have a higher rate of neurodegeneration compared to transgenic GFP controls at day 1. d HA-114 does not extend lifespan in FUSS57Δ;acs-20(tm3278) animals nor e decreases neurodegeneration phenotype at day 1 of adulthood. For lifespan assays (a, b, d), curves were generated and compared using the log-rank (Mantel–Cox) test. a: N2 n = 106; FUSS57Δ n = 104; acs-20(tm3278) n = 104; FUSS57Δ;acs-20(tm3278) n = 105. b: N2 n = 107; FUSS57Δ n = 105; acs-20(tm3278) n = 105; FUSS57Δ;acs-20(tm3278) n = 105. d: OP50 n = 210; HA-114 n = 212. For neurodegeneration assays (c, e), one-way ANOVA (c) and unpaired t test (e) were performed. For c, each condition n = 3 (25 worms per n). For c, each condition n = 4 (25 worms per n). Data are presented as mean ± SEM.
