Fig. 6: Immunofluorescence staining of fibroblasts and myofibroblasts in human colon cancer tissues.
From: An integrated map of fibroblastic populations in human colon mucosa and cancer tissues
a HHIP+ fibroblasts in CRC colon cancer tissues labeled with αSMA (red) and HHIP (green). Arrows point at the cells expressing both markers. Scale bar indicates 100 μm. b Double labeling for α-SMA (red) and RGS5 (green) in colon cancer tissues. Scale bar indicates 100 μm. c Double labeling for α-SMA (green) and CD45 (red) in colon cancer tissues. Scale bar indicates 100 μm. d Double labeling for α-SMA (green) and Podoplanin (red) in colon cancer tissues. Scale bar indicates 100 μm. e Double labeling for α-SMA (green) and Decorin (red) in colon cancer tissues. Each fluorescent channel was displayed separately. f Double labeling for α-SMA (green) and CD31 (red) in colon cancer tissues. g Double labeling for Keratin 20 (green) and α-SMA (red) in colon cancer tissues. h Double labeling for Decorin (green) and CDX2 (red) in colon cancer tissues. i Double labeling for Decorin (green) and CD31 (red) in colon cancer tissues. a–e The star indicates submucosa adjacent to cancer cells. Scale bar indicates 100 μm. j Double labeling for Keratin 20 (green) and Podoplanin (red) in colon cancer tissues. Scale bar indicates 200 μm. k Double labeling for Podoplanin (green) and CD45 (red) in colon cancer tissues. Scale bar indicates 100 μm.
