Fig. 5: Ubiquitination of Aurora B at Lys202 facilitates the formation of CPC complex. | Communications Biology

Fig. 5: Ubiquitination of Aurora B at Lys202 facilitates the formation of CPC complex.

From: The deubiquitinating enzyme complex BRISC regulates Aurora B activation via lysine-63-linked ubiquitination in mitosis

Fig. 5

a Mutation at Aurora B-K202 disturbs the interaction between Aurora B and INCENP/Survivin. HEK293T cells were transfected with the indicated plasmids and then treated with 30 ng/mL NOC for 16 h, arrested at mitosis, and the lysates were subjected to Flag-IP. The immunoprecipitates were separated and blotted with the indicated antibodies. b, d Representative confocal images of centromere localization of INCENP (red) (b) and Survivin (red) (d) in pEGFP-Aurora B or pEGFP-Aurora B-K202R transfected cells. HeLa cells were treated as described in Fig. 4e. Bar, 5 μm. c, e Box-and-whiskers plots showing the quantification of the relative intensity of INCENP or Survivin shown in b or d. Intensities were normalized to the general kinetochore marker CREST. Boxes show the upper and lower quartiles with a line at the median. Whiskers as the extent of 100% of the data. P-values were calculated by GraphPad Prism 8. Data are shown as means ± SD (n = 15 cells, and six areas for each cell were counted). **p < 0.01 versus CT, one-way ordinary ANOVA test.

Back to article page