Fig. 7: Ubiquitination of Aurora B at Lys202 is required for cell survival.

a Verification of HeLa cells that stably expressing FH-Aurora B or FH-Aurora B-K202R. Protein samples from the indicated cells were separated by SDS–PAGE, followed by IB. b Representative images of colonies formed with the indicated stable cell lines. c Quantification of the colony numbers formed in b. Data are shown as means ± SD of three independent experiments. **p < 0.01, ***p < 0.001 versus CT, Student’s t-test. d Effects of Aurora B-K202R on cell viability. CCK8 assay was performed with stable cell lines as described in a. Cell viability was normalized to the start time point. Data are shown as means ± SD of three independent experiments. **p < 0.01, ***p < 0.001 versus CT, Student’s t-test. e Flow cytometry assay was conducted to evaluate the effect of Aurora B-K202R on apoptosis. f Quantification of the apoptotic cells shown in e. Data are shown as means ± SD of three independent experiments. **p < 0.01, versus CT, Student’s t-test. g Aurora B-K202R confer sensitivity to the anti-tumor drug, taxol. CCK8 assay was performed with the indicated stable cell lines and treated with taxol for 48 h at the indicated concentrations. Data are shown as means ± SD of three independent experiments. **p < 0.01, ***p < 0.001 versus CT, Student’s t-test.