Fig. 8: Mitochondrial OxPhos defects decrease lifespan and accelerate telomere shortening. | Communications Biology

Fig. 8: Mitochondrial OxPhos defects decrease lifespan and accelerate telomere shortening.

From: OxPhos defects cause hypermetabolism and reduce lifespan in cells and in patients with mitochondrial diseases

Fig. 8

a Growth curves of control, SURF1, and Oligo-treated cells. Population doublings were determined from both live and dead cell cells at each passage. b Hayflick limit defined as the total number of population doublings achieved before division rate <0.01 divisions/day for at least two passages. c Telomere length per population doubling, d rate of telomere attrition per division, and e terminal telomere length. f Rate of epigenetic aging for control, SURF1, and Oligo-treated cells, calculated from the linear rate between days 25 and 75 (3–4 timepoints/cell line). g Average rate of epigenetic aging across all PC-based clocks. Each datapoint represents a different clock. f, g Significance values were calculated using a multiple comparison two-way ANOVA. n = 3 donors per group, 5–15 timepoints per condition for telomere length. In d, data are the slope estimate for the linear regressions in (c). Data are means ± SEM. *P < 0.05, **P < 0.01.

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