Fig. 5: Cl‾-driven base transport. | Communications Biology

Fig. 5: Cl‾-driven base transport.

From: CryoEM structures of anion exchanger 1 capture multiple states of inward- and outward-facing conformations

Fig. 5

Typical functional traces of mock a and wt-AE1 b transfected HEK293 cells. c AE1 wt (n = 10 biologically independent experiments) and single cysteine functional mutant data (depicted as percent of wt-AE1): T739C (n = 5, p = 0.4130); M741C (n = 4, p = 0.0031); G742C (n = 7, p = 0.5074); K743C (n = 7, p = 0.9817); A744C (n = 7, p < 0.0001); P747C (n = 10, p = 0.0003); G748C (n = 6, p = 0.9531); A749C (n = 8, p = 0.7563); A750C (n = 8, p < 0.0001); A751C (n = 12, p = 0.0019); Q752C (n = 6, p = 0.8550); I753C (n = 7, p = 1.0000); Q754C (n = 7, p < 0.0001); E755C (n = 8, p = 0.9993); K757C (n = 6, p = 1.0000); E758C (n = 8, p = 0.8870); Q759C (n = 7, p = 0.6288); R760C (n = 6, p < 0.0001); I761C (n = 8, p = 1.0000); S762C (n = 4, p < 0.0001); G763C (n = 4, p = 0.9968); L765C (n = 15, p < 0.0001); and L769C (n = 6, p = 0.8466). One-way ANOVA and Dunnett’s test were used to compare multiple study group means with wt-AE1. Statistically significant results differing from wt-AE1 are depicted as mean ± SEM (#p < 0.005 and *p < 0.001). Open circles represent individual data points. Source data are provided as a Source Data file.

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