Table 1 Cryo-EM data collection, refinement, and validation statistics.
(IF-IF)TMD EMDB-27267 PDB 8D9N | (IF-IF)Full-length EMDB-28055 PDB 8EEQ | (IF-OF)Full-length EMDB-27856 PDB 8E34 | ||
|---|---|---|---|---|
Data collection and processing | ||||
Magnification | 36,764 | 36,764 | 36,764 | |
Voltage (kV) | 300 | 300 | 300 | |
Electron exposure (e−/Å2) | 52 | 52 | 52 | |
Defocus range (μm) | –1.4 to –3.2 | –1.4 to –3.2 | –1.4 to –3.2 | |
Pixel size (Å) | 1.36 | 1.36 | 1.36 | |
Symmetry imposed | C2 | C1 | C1 | |
Initial particle images (no.) | 2,635,578 | 2,635,578 | 2,635,578 | |
Final particle images (no.) | 251,871 | 90,818 | 87,780 | |
Map resolution (Å) | 4.4 | 6.3 | 6.9 | |
FSC threshold | 0.143 | 0.143 | 0.143 | |
Refinement | ||||
Initial model used (PDB code) | 4YZF | 4YZF, 4KY9 | 4YZF | |
Model resolution (Å) | 4.5 | 6.5 | 7.1 | |
FSC threshold | 0.5 | 0.5 | 0.5 | |
Map sharpening B factor (Å2) | −275.87 | −283.70 | −233.43 | |
Model composition | ||||
Non-hydrogen atoms | 7,188 | 11,516 | 7,553 | |
Protein residues | 902 | 1,446 | 948 | |
Ligands | 0 | 0 | 0 | |
R.m.s. deviations | ||||
Bond lengths (Å) | 0.003 | 0.005 | 0.003 | |
Bond angles (°) | 0.594 | 1.105 | 0.714 | |
Validation | ||||
MolProbity score | 1.78 | 2.01 | 1.97 | |
Clashscore | 3 | 5.70 | 12.02 | |
Poor rotamers (%) | 0 | 0.49 | 0 | |
Ramachandran plot | ||||
Favored (%) | 94.16 | 94.61 | 94.56 | |
Allowed (%) | 5.84 | 5.39 | 5.33 | |
Disallowed (%) | 0 | 0 | 0.11 | |
