Fig. 3: Overview of the LdG6PD wt tetramer.

The tetramers of the apo a and the NADP(H)-complexed structures b are shown in the same orientation. The β-α domain (D249-K552) of subunits A/A’ and B/B’ is colored light purple and light blue, respectively. The “Rossmann-like” domain (D50-I248) of subunits A/A’ and B/B’ is colored dark magenta and navy blue, respectively. a apo structure. The helical N-terminal domain with α1 (~D11-R31) and α2 (~I36-K49) is colored gray in subunit A and A’ (N-domains N^A and N^A’) and colored dark cyan in subunit B and B’ (N-domains N^B and N^B’ with position P1). Dotted lines indicate regions with bad or missing electron density (subunit A/A’: G52-V63; subunit B/B’: K49-K62, T474-D477). b NADP(H)-complexed structure. N-domains N^A and N^A’ are colored magenta and N-domains N^B and N^B’ (position P1) are colored cyan. The connecting loop (D56-K64) is colored green, and dotted lines indicate regions with bad or missing electron density (subunit A/A’: D50-S55, Y476-R479; subunit B/B’: R51-E54). NADP(H) (yellow) and G6P (green) and residues are shown as sticks models. Important residues of the flexible loop (T471-D482) and the connected helix α14 (P481-L492) are directly or indirectly affected by the N-domains and marked with labels or arrows. The apo structure and the NADP(H)-complexed structure were used (PDBIDs: 7ZHT, 7ZHU), and G6P is taken from a superimposed structure with bound G6P (ribbon not shown, PDBID: 7ZHZ).