Fig. 5: Active site close-up of LdG6PD.
Ribbons and residues of the “Rossmann-like” domain are colored navy blue and of the β-α domain light blue. a Close-up of the NADP+ binding pocket of the LdG6PD structures (PDBID: 7ZHU) within the “Rossmann-like” domain. The NADP(H) moiety is colored yellow. Electron density map (FO-FC polder omit map) contoured at 4.0 σ for NADP(H), is shown in black. b Closeup of the G6P binding pocket. G6P is shown in two conformations. In brown, the G6P conformation from the superimposed TcG6PD structure (PDBID: 5AQ1) with the pyranose ring pointing towards the NADP(H) moiety (yellow). In green, the G6P conformation of the LdG6PD structures (PDBID: 7ZHZ). Electron density map (FO-FC polder omit map) contoured at 4.0 σ for G6P, is shown in black. Residues of the NADP+ and the G6P binding pocket, are shown in stick models, and hydrogen bonds are indicated with black dotted lines or with gray dotted lines for the G6P moiety from the TcG6PD structure.
