Fig. 7: LdG6PD’s sequential binding mechanism for the substrates NADP+ and G6P and inhibition by the product inhibitor NADPH and the dead-end inhibitor glucosamine 6-phosphate. | Communications Biology

Fig. 7: LdG6PD’s sequential binding mechanism for the substrates NADP+ and G6P and inhibition by the product inhibitor NADPH and the dead-end inhibitor glucosamine 6-phosphate.

From: Crystal structure of Leishmania donovani glucose 6-phosphate dehydrogenase reveals a unique N-terminal domain

Fig. 7

a NADP+ and b G6P, as well as c, f NADPH and d, e glucosamine 6-phosphate (GlcN 6-P) were titrated at various constant concentrations of the substrate G6P or cosubstrate NADP+. The primary double reciprocal plots of [U/100 mg]−1 against [G6P]−1 and [NADP+]−1, respectively, are shown. Plots a and b intercept left of the ordinate, indicating a sequential binding mode of the substrates. Plots c, e, and f intercept on the ordinate, indicating a competitive inhibition of NADPH against NADP+ and G6P and of GlcN 6-P against G6P, but not against NADP+. d Representative graphs from three independent replications are shown.

Back to article page