Fig. 6: Competition of α-Bgtx and WTX and its mutants with TRITC-labeled α-Bgtx for binding to α7-nAChR expressed in HEK293 cells.

a Data on competition of α-Bgtx with TRITC-labeled α-Bgtx presented as median fluorescence intensities (MFI) ± SEM (n = 5 independent experiments). The Hill equation (y = A0 + A1/(1 + ([Toxin]/IC₅₀)^nH)) was fitted to data. b Data on competition of WTX and its mutants with TRITC-labeled α-Bgtx were normalized to the TRITC-α-Bgtx-binding in absence of WTX or its mutants and shown as mean ± SEM (n = 5 independent experiments). The Hill equation (y = 100%/(1 + ([Toxin]/IC₅₀)^nH)) was fitted to normalized data. Individual datapoints for this dataset are given in Supplementary Fig. 9. **p < 0.01 and ***p < 0.001 indicate the significant difference between WTX and WTX[R18A, E21A] groups by two-sided t-test with Holm-Sidak correction for multiple comparisons.