Fig. 2: Evidences of interaction between CKRC3/TCU2 and NBC to form a NatB for maintaining endogenous auxin levels. | Communications Biology

Fig. 2: Evidences of interaction between CKRC3/TCU2 and NBC to form a NatB for maintaining endogenous auxin levels.

From: Significance of NatB-mediated N-terminal acetylation of auxin biosynthetic enzymes in maintaining auxin homeostasis in Arabidopsis thaliana

Fig. 2

a Y2H test in vitro, with pGBKT7-53 and pGADT7-T as positive, pGBKT7-lam and pGADT7-T as negative controls, respectively. b Interaction between CKRC3 and NBC in vivo revealed by Co-IP test after transiently co-expressing 35S::Myc-CKRC3 and 35S::HA-NBC in N.benthamiana leaves. The nuclear protein ICE1 was used as negative control. c–e Plant phenotypes at 7 DAG; bar = 5 mm, n ≥ 12. Data are presented as mean ± SD, different capital letters indicate significant differences at P < 0.01 according to ANOVA followed by Tukey’s multiple comparison tests. f GUS staining of DR5::GUS marker lines, bar = 100 μm. g Quantification of root gravitropic responses (n ≥ 39). h, j Rosettes and fresh weight of 30 DAG plant (n = 31). Data are presented as mean ± SD, different capital letters indicate significant differences at P < 0.01 according to ANOVA followed by Tukey’s multiple comparison tests. i Primary root length at 7 DAG on MS medium with increasing concentrations of IAA (n ≥ 15). Data are presented as mean ± SD.

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