Fig. 4: Chromosome segregation defects in Profilin 1-deficient RPE1 cells.
From: Profilin 1 deficiency drives mitotic defects and reduces genome stability
a Schematic of the experiment created in BioRender (https://biorender.com/) by F.S.d.C. as a registered user. Below, representative immunofluorescence images of mitotic PFN1-KO RPE1 cells showing b chromosome misalignment (arrowhead) at the metaphase plate, c multipolar spindle formation, d a chromosome bridge (arrowhead) persisting in late anaphase, e telophase with a lagging chromosome (arrowhead). Cells were stained for microtubules (α-tubulin, green) and DNA (Hoechst 33342, blue); scale bars 5 µm. In each panel, the overlay shows α-tubulin/DNA staining. Control metaphase, anaphase, and telophase images of dividing RPE1 cells are illustrated in Fig. 1. f Quantification (%) of cells with misaligned chromosomes; **P = 0.0096, *P = 0.0411. g Quantification (%) of cells with multipolar spindles; *P = 0.0305, *P = 0.0259. h Quantification (%) of cells with anaphase bridges; ***P = 0.0003, ***P = 0.0009. i Quantification (%) of cells with lagging chromosomes; ns P = 0.2457, *P = 0.0345. Data in f–i are shown as mean ± s.e.m. of three independent experiments (n = 2 biological replicates for WT, 3 for PFN1+/−, 3 for PFN1−/− cells); dots represent the value of each experiment (scoring 911, 825 and 616 total mitoses in WT, PFN1+/− and PFN1−/− samples, respectively); range of number of cells used for each individual experiment: 70-266 WT, 30-189 PFN1+/−, 24-116 PFN1−/−. Ordinary one-way ANOVA performed.
