Fig. 1: Generation and validation of the Emx1-NuTRAP mouse as a tool for generating sequencing-grade, neuron-specific DNA and translating mRNA from a single hippocampal homogenate. | Communications Biology

Fig. 1: Generation and validation of the Emx1-NuTRAP mouse as a tool for generating sequencing-grade, neuron-specific DNA and translating mRNA from a single hippocampal homogenate.

From: Early-life exercise primes the murine neural epigenome to facilitate gene expression and hippocampal memory consolidation

Fig. 1

a Schematic representation of the Emx1-NuTRAP mouse generation and workflow of the Simultaneous INTACT and TRAP (“SIT”) protocol. Created with BioRender.com. b Immunofluorescence imaging of the CA1 region of the hippocampus at ×20 objective and b′ ×60 objective after incubation with an mCherry antibody . c Immunofluorescence imaging of the CA3 region of the hippocampus at ×20 objective and c′ ×60 objective after incubation with an mCherry antibody . d Immunofluorescence imaging of the DG region of the hippocampus at ×20 objective and d′ ×60 objective after incubation with an mCherry antibody . Scale bars are set to 50 µm for all images b–d. e Flow cytometry for EGFP, Thy1, and S100β (15.3% of cells Thy1 + /EGFP + and 0.13% of cells S100β+/EGFP+). f Heatmap of differentially expressed neuronal and non-neuronal cell type markers from RNA-seq data comparing TRAP-isolated RNA from hippocampus of Emx1-NuTRAP mice vs hippocampal mRNA isolated from wild-type mice. (Abbreviations: NPC neural progenitor cell, Oligo oligodendrocyte).

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