Fig. 5: ELE alters H4K8ac and H3K27me3 occupancy at differentially expressed genes.

a Venn diagram of gene peaks identified in CUT&RUN-seq for H4K8ac and H3K27me3 by SEACR (top 1% of peaks FDR < 0.1) overlapped with genes upregulated (a) and downregulated (a′) in RNA by ELE (n = 2 sedentary mice and n = 3 ELE mice). b CUT&RUN-seq SEACR peak calls at genes that overlap with genes differentially expressed by ELE as determined by TRAP-seq. c Stacked bar of the relative distribution of peak calls at various genomic regions identified by CUT&RUN-seq called using SEACR. d Representative track of CUT&RUN-seq data around Prox1 showing differential peak height between ELE and sedentary conditions for H4K8ac (top) and H3K27me3 (bottom).