Fig. 6: ELE may prime hippocampal gene expression supporting memory consolidation via alterations in H4k8ac and H3k27me3 occupancy.

a Experimental design diagram. Created with BioRender.com. b Venn diagram representing upregulated genes relative to mice without ELE or learning in either ELE, ELE and 3 min OLM training, Sed and 3 min OLM training, and Sed and 10 min OLM training. b′ Pie chart showing the relative percentage of genes that have new H4K8ac presence or H3K27me3 loss as a result of ELE in specific groups identified in the b labeled cGAME and cGAMES. c Same as b but for downregulated genes relative to mice without ELE. c′ Pie chart of downregulated genes in the cGAME or cGAMES groups that have new H3K27me3 or new loss of H4K8ac as a result of ELE. d Z-score based heatmap of genes in the cGAMES and cGAME groups that have new H4K8ac or H3K27me3 presence or loss as a result of ELE. Expression values used to calculate the Z-scores were relative to mice that did not experience OLM training or ELE. e Upstream regulators identified by IPA of the genes represented by d. e′ Upstream regulators identified by IPA of the genes in the cGAME (up and downregulated) group that are unique to that when only those are included in the IPA analysis. e″ Upstream regulators identified by IPA of the genes in the cGAME and cGAMES (up and downregulated) group when the genes in those groups are put through IPA together. (asterisk indicates that the activated or inhibited indicator is registered from the IPAs based on the z-score for that regulator’s expression and not directly indicated based on software prediction).