Fig. 1: The blockade of TAM receptors improves M1 responses to immune T cells. | Communications Biology

Fig. 1: The blockade of TAM receptors improves M1 responses to immune T cells.

From: Axl receptor induces efferocytosis, dampens M1 macrophage responses and promotes heart pathology in Trypanosoma cruzi infection

Fig. 1

BMDMs from B6 mice were treated with (closed circles) or without (open circles) the TAM inhibitor Mer-Ig and then cocultured in triplicate with T cells from T. cruzi-infected B6 mice (15 dpi) for 48 h. Macrophages were washed to remove T cells, collected, and stained. Gated F4/80+ cells were further analysed for a, b surface CD301 and intracellular IL-12p35 expression or control isotypes (IgG2b and mIgG1); c, d intracellular iNOS and Arg1 expression or isotype controls (IgG2a and sIgG). b, d The results are expressed as the means and SEM of n = 3 technical replicates and represent two independent experiments. Significant differences are indicated for **P < 0.01, and ****P < 0.0001, as analysed by one-way ANOVA followed by Bonferroni posttest of selected pairs of data (macrophages cultured alone versus those cocultured with T cells; macrophages cocultured with T cells in the absence of treatment versus those treated with Mer-Ig).

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