Fig. 1: Dysregulated genes and processes in cardiomyocytes and endothelial cells from β-cat^Δex3 hearts.

a Representative uniform manifold approximation and projection (UMAP) plot after scRNA-seq and data integration of cardiac cells isolated from control (673 cells) or β-cat^Δex3 (601 cells) hearts. Six cell clusters were identified and exclusively classified into cardiomyocytes (CM), endothelial cells (EC), fibroblast (FB), pericytes (PC), macrophage (MC) and neural-like cells (NLC) in both conditions. b UMAP plot of cardiomyocyte subclusters CM1, CM2) with respective hypertrophic stress scores as defined by the expression of Acta1, Nppa, Nppb and Ankrd1. c Dot plot depicting expression of selected cardiac stress and Wnt signaling target transcripts in cardiomyocytes from β-cat^Δex3 and control hearts as indicated. d Selected top categories from GO biological processes enrichment of upregulated DEGs in CM1 and CM2 of β-cat^Δex3 representing transcriptional responses. e Dot plot depicting expression of selected developmental and angiogenesis transcripts in endothelial cells (EC1 and EC2) from β-cat^Δex3 and control hearts as indicated. f Selected top categories from GO biological processes enrichment of upregulated DEGs in EC1 and EC2 of β-cat^Δex3 representing transcriptional responses in endothelial clusters. GO enrichment represents –log10 p-value (p-value <0.05) and term fusion was applied. g Increased Ub-protein abundance in β-cat^Δex3 hearts versus control. Staining-free gel is provided as loading control.