Fig. 2: Increased cardiac EV cargo secretion in β-cat^Δex3 hearts.

a Nanoparticle tracking analyses (NTA) confirmed isolation of extracellular vesicles with a mean size of 160.0 ± 69 nm. Visualization of the purified vesicles by hydrophilic fluorescence analog of cholesterol (Chol-PEG-KK114) staining. b Electron microscopy confirmed the isolation of round and cup-shaped particles, typical for exosomal vesicles less than 200 nm in size. c Western blot showing expression of the exosome marker CD81 in the higher speed-centrifugation fraction (P100) from mouse hearts and the absence of Golgi-marker GM130 and endoplasmic reticulum marker Calnexin, which were present in the cell pellets and lower-speed centrifugation (S100). d Western blot showing increased exosomal marker TSG101 in EV isolated from β-cat^Δex3 hearts versus control, as well as showing absence of Calnexin, GAPDH and Vinculin expression in both conditions upon equally amount of loaded proteins as visualized by the stain free gel image. e GO biological processes distribution of enrichment analysis of total proteomic data (573 proteins) obtained from isolated vesicles of β-cat^Δex3 hearts and control confirming an association of vesicle contained proteins with “extracellular exosomes” (n = 2 (each a pool of 2-3 hearts) biological replicates and technical triplicates for NTA and MS analysis, n = 4, biological replicates (for Western blots). GO enrichment represents –log10 p-value (p-value <0.05) and term fusion was applied.