Table 1 The specific activity of the recombinant IsPETase.

From: Improving the activity and thermostability of PETase from Ideonella sakaiensis through modulating its post-translational glycan modification

Enzyme

TPA (mM)

MHET (mM)

Percentage of TPA (%)

specific activity (U/mg)

IsPETase-Ec

0.0023 ± 0.0004

0.0039 ± 0.0004

36.84 ± 1.72

1.0 ± 0.17

IsPETase-Pp

0.2492 ± 0.0061

0.0386 ± 0.0011

86.60 ± 0.06

20.6 ± 0.74

partial-deglyco IsPETase-Pp

0.8427 ± 0.0154

0.0469 ± 0.0012

94.72 ± 0.02

62.4 ± 1.64

  1. The results are presented as the means ± SDs of three independent experiments. To analyze the enzyme activity, 0.4 mg of enzyme and 3 mg of amorphous PET were incubated in 1 mL of 50 mM glycine-NaOH buffer (pH 9.0) at 30 °C for 6 h.
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