Table 2 IsPETase kinetics for MHET and BHET.

From: Improving the activity and thermostability of PETase from Ideonella sakaiensis through modulating its post-translational glycan modification

 

MHET

BHET

Enzyme

KM

kcat

kcat/KM

KM

kcat

kcat/KM

partial-deglyco IsPETase-Pp

0.84 ± 0.09

37.99 ± 4.14

45.23

0.88 ± 0.17

40.14 ± 7.55

45.61

IsPETase-Ec

1.04 ± 0.07

15.61 ± 1.03

15.01

0.30 ± 0.09

12.81 ± 3.81

42.70

  1. The results are presented as the means ± SDs of three independent experiments.
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