Fig. 3: ZNF185 knockdown induces stress fiber formation.
From: ZNF185 prevents stress fiber formation through the inhibition of RhoA in endothelial cells
a. ZNF185 interacts with actin. Myc-ZNF185-HA was overexpressed in HUVECs. One hour after forskolin (10 µM) treatment, HA-beads were used for coimmunoprecipitation. Representative blots are shown (n = 3). Densitometric analysis of immunoprecipitated endogenous actin. Two-sided Student’s t test, **p < 0.01 (n = 3). b–d ZNF185 knockdown induces stress fiber formation and discontinuous cell–cell junctions in a confluent HUVECs monolayer. b Immunofluorescence staining of VE-cadherin (magenta) and actin (green). Arrows indicate discontinuous zig-zag junctions. Arrowheads indicate intercellular gaps. Representative images are shown (n = 3, Supplementary Fig. 6). Scale bars, 25 µm. c Intensity of stress fibers at the yellow lines in Fig. 3b was quantified. d The bar graph shows total amount of stress fiber intensity in Fig. 3c. Two-sided Student’s t test, *p < 0.05 (n = 3). Data are presented as the mean ± standard error. Ctrl control, Fsk forskolin.
