Fig. 8: N-terminal ZNF185 lacking PKA-interacting domain is insufficient to inhibit stress fiber formation.
From: ZNF185 prevents stress fiber formation through the inhibition of RhoA in endothelial cells
a Thrombin-induced stress fiber formation and disruption of adherens junction are inhibited by ZNF185-Δexon 11,12. Thrombin (10 U/mL) was added to HUVECs overexpressing Myc-ZNF185-HA or Myc-ZNF185-Δexon 11,12-HA for 1 h. Immunofluorescence staining of actin (green) and VE-cadherin (magenta). Arrowheads indicate intercellular gaps. Representative images are shown (n = 3). Scale bars, 25 µm. b Schematic representation of N- and C-terminal of ZNF185. c Myc-N-terminal ZNF185 (green) is translocated to the membrane region after the treatment of forskolin for 1 h. C-terminal ZNF185-HA (magenta) is dispersed throughout the cytosol (n = 3). Scale bars, 25 µm. d Inhibition of RhoGDIα phosphorylation by ZNF185 knockdown is not rescued by overexpression of N-terminal ZNF185. e N-terminal ZNF185 does not rescue stress fiber formation by ZNF185 knockdown. Immunofluorescence staining of actin (green) and VE-cadherin (magenta). Representative images are shown (n = 3). Scale bars, 25 µm.
