Table 1 Characteristics of single aggregates formed from non-infected and fungal-infected diatom cultures.

From: Fungal parasitism on diatoms alters formation and bio–physical properties of sinking aggregates

Parameter

Non-infected aggregate

N

Fungal-infected aggregate

N

Unit

P-value

Method/Equation

Set I

       

Diameter d

2.4 ± 1.3

(1.1–4.9)

12

2.4 ± 1.0

(1.3–4.7)

12

mm

0.93

Image analyses

Porosity φ

0.9993 ± 0.0002 (0.9990–0.9997)

12

0.9995 ± 0.0002 (0.9993–0.9997)

12

-

0.56

Eq.4

Excess density ∆ρ

0.8 ± 0.3

(0.4–1.3)

12

0.6 ± 0.1

(0.4–0.9)

12

mg cm−3

0.009

Eq.5

Drag coefficient CD

13.1 ± 11.4

(2.2–43.2)

12

14.9 ± 9.8

(2.9–36.9)

12

-

0.69

Eq.6

Reynolds number Re

6.8 ± 8.7

(0.6–28.2)

12

4.6 ± 5.5

(0.7–18.1)

12

-

0.46

Eq.7

Settling velocity U

201 ± 148

(52–534)

12

144 ± 103

(52–363)

12

m d−1

0.04

(curve fit)

Settling column

Fractal dimension D3

2.37 ± 0.30

12

2.64 ± 0.24

12

-

0.005

(curve fit)

Eq.8

Set II

       

POC contenta

6.7 ± 2.8

(3.6–11.4)

9

8.0 ± 5.3

(1.6–15.4)

9

µg C agg−1

0.52

Carbon analyzer

C-specific respiration

0.16 ± 0.08

(0.08–0.31)

9

0.34 ± 0.08

(0.22–0.45)

9

d−1

0.0002

Oxygen sensor

Set III

       

Bacterial colonizationb

2.7 ± 0.5 × 104

(1.8–3.3 × 104)

9

12.3 ± 0.8 × 104

(11.0–13.3 × 104)

9

agg−1

<0.0001

Microscopy

Set IV

       

Mass density aggregates ρs-agg

1.284 ± 0.003

(1.280–1.289)

6

1.258 ± 0.004

(1.253–1.262)

4

g cm−3

<0.0001

Density gradient

Set V (Fungal-infected treatment)

Ambient water

 

Aggregate

    

Infection prevalence

42 ± 3

(37–45)

6

(1 mL)

71 ± 3

(66–76)

9

%

0.0001

Microscopy

Co-cultures (Single Synedra cellsc)

Non-infected cells

 

Infected cells

(incl. sporangium)

    

Mass density cells ρs-cells (single diatom cells)

1.269 ± 0.001d

(1.268–1.271)

3

(flasks)

1.251 ± 0.004

(1.249–1.256)

3

(flasks)

g cm−3

0.008

Density gradient

Cell biovolume

1446 ± 265

(855–2161)

50

(cells)

1688 ± 238

(1217–2701)

20

(cells)

µm−3

0.005

Microscopy

  1. The similar-sized aggregates were picked individually after their formation in rotating cylinders. Aggregates were grouped into five sets (set I–V, each with 4–12 replicates) since not all parameters could be measured on the same aggregate. N denotes the number of aggregates, if not specified differently. Bold P-values indicate statistically significant differences between the non-infected and fungal-infected treatments.
  2. aPOC – particulate organic carbon.
  3. bassuming 20,000 diatom cells per aggregate since we counted 22,843 ± 34,443 Synedra cells per aggregate (d = 2.1 ± 0.8 mm in set III, N = 18).
  4. csampled directly from the co-cultures (not from the rotating cylinders).
  5. dmass density is given for non-infected Synedra cells that were sampled from the fungal-infected treatment, while it was similar for non-infected Synedra cells in the non-infected treatment (1.270 ± 0.006 g cm−3, range 1.264–1.276 g cm−3, P = 0.97, N = 3).