Table 1 Characteristics of single aggregates formed from non-infected and fungal-infected diatom cultures.
From: Fungal parasitism on diatoms alters formation and bio–physical properties of sinking aggregates
Parameter | Non-infected aggregate | N | Fungal-infected aggregate | N | Unit | P-value | Method/Equation |
---|---|---|---|---|---|---|---|
Set I | |||||||
Diameter d | 2.4 ± 1.3 (1.1–4.9) | 12 | 2.4 ± 1.0 (1.3–4.7) | 12 | mm | 0.93 | Image analyses |
Porosity φ | 0.9993 ± 0.0002 (0.9990–0.9997) | 12 | 0.9995 ± 0.0002 (0.9993–0.9997) | 12 | - | 0.56 | Eq.4 |
Excess density ∆ρ | 0.8 ± 0.3 (0.4–1.3) | 12 | 0.6 ± 0.1 (0.4–0.9) | 12 | mg cm−3 | 0.009 | Eq.5 |
Drag coefficient CD | 13.1 ± 11.4 (2.2–43.2) | 12 | 14.9 ± 9.8 (2.9–36.9) | 12 | - | 0.69 | Eq.6 |
Reynolds number Re | 6.8 ± 8.7 (0.6–28.2) | 12 | 4.6 ± 5.5 (0.7–18.1) | 12 | - | 0.46 | Eq.7 |
Settling velocity U | 201 ± 148 (52–534) | 12 | 144 ± 103 (52–363) | 12 | m d−1 | 0.04 (curve fit) | Settling column |
Fractal dimension D3 | 2.37 ± 0.30 | 12 | 2.64 ± 0.24 | 12 | - | 0.005 (curve fit) | Eq.8 |
Set II | |||||||
POC contenta | 6.7 ± 2.8 (3.6–11.4) | 9 | 8.0 ± 5.3 (1.6–15.4) | 9 | µg C agg−1 | 0.52 | Carbon analyzer |
C-specific respiration | 0.16 ± 0.08 (0.08–0.31) | 9 | 0.34 ± 0.08 (0.22–0.45) | 9 | d−1 | 0.0002 | Oxygen sensor |
Set III | |||||||
Bacterial colonizationb | 2.7 ± 0.5 × 104 (1.8–3.3 × 104) | 9 | 12.3 ± 0.8 × 104 (11.0–13.3 × 104) | 9 | agg−1 | <0.0001 | Microscopy |
Set IV | |||||||
Mass density aggregates ρs-agg | 1.284 ± 0.003 (1.280–1.289) | 6 | 1.258 ± 0.004 (1.253–1.262) | 4 | g cm−3 | <0.0001 | Density gradient |
Set V (Fungal-infected treatment) | Ambient water | Aggregate | |||||
---|---|---|---|---|---|---|---|
Infection prevalence | 42 ± 3 (37–45) | 6 (1 mL) | 71 ± 3 (66–76) | 9 | % | 0.0001 | Microscopy |
Co-cultures (Single Synedra cellsc) | Non-infected cells | Infected cells (incl. sporangium) | |||||
---|---|---|---|---|---|---|---|
Mass density cells ρs-cells (single diatom cells) | 1.269 ± 0.001d (1.268–1.271) | 3 (flasks) | 1.251 ± 0.004 (1.249–1.256) | 3 (flasks) | g cm−3 | 0.008 | Density gradient |
Cell biovolume | 1446 ± 265 (855–2161) | 50 (cells) | 1688 ± 238 (1217–2701) | 20 (cells) | µm−3 | 0.005 | Microscopy |