Fig. 2: Structural features of azurin in complex with p53. | Communications Biology

Fig. 2: Structural features of azurin in complex with p53.

From: Structural basis of bacterial effector protein azurin targeting tumor suppressor p53 and inhibiting its ubiquitination

Fig. 2

a Cartoon rendering of the azurin-p53 structure and expanded views of the azurin-p53 interface is highlighted. Residues contributing to intermolecular contacts are presented as ball-and-stick. Intermolecular hydrogen bonds are shown as broken lines colored in cyan. b Immunoblots assay showing the effect of point mutations on the binding affinity of azurin and p53-DBD. This assay was performed with anti-Flag beads which specifically binds to Flag-DBD or its mutants to pull down strep-azurin in solution, and the elution was blotted with anti-flag and anti-strep antibodies, respectively. The intensity of the blot of azurin represents the relative affinity of the azurin and p53-DBD. c Chemical crosslinking mass spectrometry analysis of the azurin-p53 complex. The crosslinking reagents used are specifically react with lysine. All the Nz atom of lysine residues are shown and highlighted in blue. The crosslinked pairs captured are connected with yellow broken lines.

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