Fig. 5: Affinity-enhancing mutations promote p53-regulated apoptosis. | Communications Biology

Fig. 5: Affinity-enhancing mutations promote p53-regulated apoptosis.

From: Structural basis of bacterial effector protein azurin targeting tumor suppressor p53 and inhibiting its ubiquitination

Fig. 5

a Flow cytometry profiles of cells treated with different type of reagents, containing phosphate buffer as control, azurin and its four mutants. b Statistics of the apoptosis rate of cells treated with azurin or its mutants obtained from three paralleled experiments. Data are presented as dot, n = 3 independent replicates. Error bars indicate mean ± SEM. P-values were determined by one way ANOVA followed by Dunnett’s multiple comparisons test using PBS group as a control, **P < 0.001, ***P < 0.0001. c Immunoblotting analysis of protein level in cells treated with azurin and the affinity-enhancing mutants. The antibodies for each protein are anti-his antibody for azurin, anti-p53 antibody for p53, anti-p21 antibody for p21, anti-BAX antibody for BAX and anti-beta-actin antibody for beta-actin.

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