Fig. 3: Genome-wide deposition of m6dA by the bacterial CcrM MTase leads to the differential expression of multiple genes. | Communications Biology

Fig. 3: Genome-wide deposition of m6dA by the bacterial CcrM MTase leads to the differential expression of multiple genes.

From: Genome-wide deposition of 6-methyladenine in human DNA reduces the viability of HEK293 cells and directly influences gene expression

Fig. 3

a–c Scatter plots of RNA-seq data normalized by DeSeq2 (normalized counts). Pair-wise comparisons of ‘no dox CcrM-WT’ versus ‘10d dox CcrM-WT’ (panel a), ‘10d dox CcrM-inactive’ (D39A) versus ‘10d dox CcrM-WT’ (panel b), and ‘no dox CcrM-WT’ versus ‘10d dox CcrM-inactive’ (D39A) (panel c). Red dots represent differentially expressed genes identified by DeSeq2 (p < 0.05 after Benjamini–Hochberg p-value correction for multiple testing). A pseudo-count of 0.1 was added to all values. d Venn diagram showing the overlap of differentially expressed (DE) genes after 10 days of CcrM expression in comparison to ‘no dox CcrM’, and ‘10d dox CcrM D39A’.

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