Fig. 2: Characteristics of circCEBPZOS.

a Schematic diagram of the ring structure of circCEBPZOS. b Agarose electrophoresis assay to amplify the specific products of circCEBPZOS from both cDNA and gDNA templates by using convergent and divergent primers. GAPDH served as a control. c Reverse shear site of circCEBPZOS was confirmed by Sanger sequencing of the amplified product of circCEBPZOS; d qRT–PCR was used to detect the expression of circCEBPZOS by using cDNA as a templates with the RNA digested by RNA exonuclease R before reverse transcription. GAPDH served as the negative control. Data are mean ± SD. **P < 0.01, RNase R + group vs. RNase R- group.