Fig. 3: circCEBPZOS promotes angiogenesis of CMECs and proliferation and migration of VSMCs.

a qRT–PCR analysis was used to detect the expression of circCEBPZOS in the coincubation with EVs harboring with Over-circCEBPZOS or Sh-circCEBPZOS, GAPDH served as control; b Capillary-like structure analysis by tube formation assay in the Over-circCEBPZOS or Sh-circCEBPZOS group compared with the NC group, Bar = 200 μm; c CCK-8 assay was used to detect the viability of VSMCs coincubated with exosomes harboring Over-circCEBPZOS or Sh-circCEBPZOS at 24 h, 48 h and 72 h; d Flow cytometry was used to detect the cell cycle of VSMCs coincubation with exosomes harboring with Over-circCEBPZOS or Sh-circCEBPZOS; e Transwell analysis was used to detect the migration of VSMCs coincubated with EVs harboring with Over-circCEBPZOS or Sh-circCEBPZOS, Bar = 200 μm. Data are mean ± SD. **p < 0.01, Over-circCEBPZOS group vs NC group; ##p < 0.01, Sh-circCEBPZOS vs NC group.