Fig. 7: miR-1178-3p-mediated PDPK1 regulated angiogenesis of CMECs and proliferation and migration of VSMCs.

a, b Expression of PDPK1 was detected by qRT–PCR and western blotting by overexpressing or suppressing of PDPK1 in CMECs, respectively, GAPDH served as a control; cc Tube formation analysis was used to detect capillary-like structures in the PDPK1 overexpression or suppression group and rescued by miR-1178-3p; Bar = 200 μm; d CCK-8 assay was used to detect the viability of VSMCs in the PDPK1 overexpression or suppression of group and rescued by miR-1178-3p at 24 h, 48 h and 72 h; E Flow cytometry was used to detect the cell cycle of VSMCs in the PDPK1 overexpression or suppression group and rescued by miR-1178-3p; f Migration of VSMCs was detected by Transwell assay when PDPK1 overexpressed or suppressed and rescued by miR-1178-3p, Bar = 200 μm. Data are mean ± SD. **P < 0.01, Over-PDPK1 group vs. NC group; ##P < 0.01, Sh-PDPK1 group vs. NC group.